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On 23 December 1588, Henri I, Duke of Guise was assassinated by the Quarante Cinq serving King Henri III. The event was one of the most critical moments of the French Wars of Religion. The duke had achieved, since 1584, considerable power over the kingdom of France, through his alliance with the Ligue movement, which he had co-opted for the cause of resisting the king's chosen successor to the Crown of Navarre, and thus France: the future Henri IV — a Protestant. Despite some effort to resist Guise and the ligue, Henri III had been forced by his weak position to accede to their continued demands. After the Day of the Barricades in May 1588, the ligue expelled Henri from Paris, and Henri was forced to make Guise lieutenant general of the kingdom, call an Estates General and sign an Edict of Union in July which prohibited Navarre from succeeding to the throne and outlawed Protestantism in France. Increasingly unable to bear the humiliations Guise and the ligue forced upon him, he was further outraged by the Estates General. The body — largely ligueur-dominated — rejected his attempt to chastise Guise for forming associations, diverted tax income to Guise's brother Mayenne and rejected all compromise with the king.
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